Journal: bioRxiv
Article Title: Dietary glutamine supplementation alleviates age-related cardiac dysfunction by reducing elevated H3K27me3
doi: 10.1101/2025.05.16.654624
Figure Lengend Snippet: H3K27me3 is elevated in the aged mouse and human myocardium. A) Histone methylation in young (12-weeks) and aged mouse myocardium (68-weeks) (n=6). B) Histone H3K27me3, H3K4me3 and H3K9me3 in young (12-weeks) and very aged mice (85-weeks) (n=4-5). C) H3K27me3 visualised throughout the young and aged mouse heart (n=4). D) H3K27me3 in young (≤40 years) and aged human (>40 years) myocardium observed by immunoblotting of tissue samples (n=4-6) (E) or immunofluorescence staining of cardiac tissue microarrays (n=9-10) (F). *P<0.05; **P<0.01; ***P<0.005 by Student t test.
Article Snippet: Human cardiac tissue microarrays (Biomax Inc, HEN241a) and paraffin-embedded young (12-week) and aged (68-week) mouse heart sections (Jackson Laboratory, #TSH0022) were immunostained for H3K27me3.
Techniques: Methylation, Western Blot, Immunofluorescence, Staining